Journal: PeerJ

Article Title: Comparison of gene expression profiles between human erythroid cells derived from fetal liver and adult peripheral blood

doi: 10.7717/peerj.5527

Figure Lengend Snippet: (A) CD34 + immuno-enriched stem cells (FL, solid line; AB, dashed line) were grown in liquid culture according to the two-step protocol. Note that the purity of immuno-enriched cells was not assessed before expansion. Total cells were counted daily from day 4 to day 8 of culture. Mean values of fold-change relative to day 4 of culture with the standard deviation for five experiments are shown. (B) Flow cytometry analysis for transferrin receptor (CD71) and glycophorin A (GPA) surface expression on culture day 8. Flow cytometric gates are denoted as CD71 − /GPA − (lower left quadrant), CD71 + /GPA − (upper left quadrant), CD71 + /GPA + (upper right quadrant) and CD71 − /GPA + (lower right quadrant). (C) Morphology of cells harvested at culture day 8.

Article Snippet: Mononuclear cells were isolated from the fetal liver cell suspension and adult healthy peripheral blood by centrifugation on a density gradient of 1.077 g/mL (Lymphoprep, Axis-Shield PoC AS, Oslo, Norway) and subsequently selected for CD34 + cells using the positive immune-magnetic selection method (CD34 MicroBead Kit, Miltenyi Biotech Inc.) according to the manufacturer’s recommendations.

Techniques: Standard Deviation, Flow Cytometry, Expressing